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Anti-STn Antibody

Anti-STn

Description:

Dysregulation of cellular glycosylation is a common characteristic of many cancers (1). The STn antigen (Neu5Acα2-6GalNAcα1-O-Ser/Thr), also known as the sialyl-Tn antigen, is up-regulated in a wide variety of cancers (2). It is formed from the Tn antigen (GalNAcα1-O-Ser/Thr) through the action of the ST6GalNAc family of enzymes (3,4). STn-Keyhole Limpet Hemocyanin conjugate has been tested as an anti-cancer vaccine (5). The specificity of the anti-STn monoclonal antibody was shown by binding to bovine submaxillary mucin (BSM), which presents the STn antigen, and not to asialo-BSM (ref 2 and Figure 1 panel B). This antibody was previously marketed under HB-STn (3,4).

  1. Hakomori, S. (1996) Cancer Res. 56, 5309.
  2. Kjeldsen, T. et al. (1988) Cancer Res. 48, 2214.
  3. Marcos, N.T. et al. (2004) Cancer Res. 64, 7050.
  4. Julian, S. et al. (2001) Glycocon. J. 18, 883.
  5. Miles, D. et al. (2006) J. Biol. Chem. 281, 3586.

Preparation:  Monoclonal antibodies were produced from the mouse hybridoma 3F1 clone (HB-STn).

Purification:  This IgG1 was purified by Protein G affinity chromatography.

Catalog #:  SBH-ASTn

Formulation:  Sterile filtered solution PBS, at a stock concentration of 640 ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Reconstitution:  N/A

Specificity Assay:  To create asialo bovine submaxillary mucin (aBSM), 30mg of BSM (Sigma M3895) was treated treated with 5U of Neuraminidase (Sigma N2876) for 2 hrs at 37C.  Double dilution ELISAs were carried out by coating 96 well plates with a 1:2 serial dilution of 10ng/ml BSM or aBSM, blocking and incubating with a 1:4 serial dilution of 10ug/ml anti-STn (SBH-STn).  After washing, plates were incubated with a goat-anti-mouse (IgG+IgM)-HRP conjugate (Thermo 31446) at 1:1000 dilution.  After washing, plates were developed with 2x ABTS (Southern Biotech 0202-01).  Net OD was measured at 405 – 650 nm.  As low as 2ng/ml SBH-STn MAb could detect BSM coated at 25 ng/ml, with no detectable binding to aBSM (see Figure 1 panel (B) above).

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA

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