Recombinant Human Core 3 synthase/B3GNT6 (β-1,3-N-Acetylglucosaminyltransferase 6), soluble fragment
β1,3Nacetylglucosaminyltransferase 6 (β3GnT6) transfers N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to N-acetylgalactosamine (GalNAc). β3GnT6 is also known as the core 3 synthase, because it creates the core 3 oligosaccharide structure in the mucin-type O-glycan, GlcNAcβ13GalNAcα1serine/threonine (1). Core 3 synthase is a type II transmembrane protein, and is localized to stomach, small intestine and colonic tissues (1). It has long been known that synthesis of the core 3 structure is down-regulated in colonic cancer cells (2) and it has been shown that the expression of β3GnT6 is also down-regulated (1). Colonic cancer cells transfected with β3GnT6 have reduced metastatic potential compared to non-transfected cells, and when injected into mice show decreased tumor formation (3). Pancreatic cancer cells transfected with β3GnT6 show a similar phenotype, which has been attributed to the addition of core3 O-glycans to α2β1 integrin (4).
- Iwai, T. et al. (2002) J. Biol. Chem. 277:12802.
- Vavasseur , F. et al. (1995) Glycobiology 5:351.
- Iwai, T. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4572.
- Lee, S.H. et al. (2009) J. Biol. Chem. 284:17157.
Catalog #: SBH-003- B3GNT6
Source : Insect Cells, Accession # Q6ZMB0
Formulation: Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 100 ug/mL.
Stability: Stable for 4 weeks at 4C. Stable for 6 months at -80C. Avoid repeated freeze-thaw cycles.
Purity: Greater than 95% by SDS-PAGE
Biological Activity: Measured by transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to 4-Nitrophenyl-N-acetylgalactosamine (4-NP-GalNAc, Sigma N4264), as measured with a phosphatase-coupled assay. Coupling assay reactions contained: 25mM Tris pH 7.5, 150mM NaCl, 10mM MnCl2, 5mM CaCl2, 20% DMSO, 0.2mM UDP-GlcNAc, 0.6mM 4-NP-GalNAc, 1.67ug/mL ENTPD3/CD39L3, and 6ug/mL B3GnT6. Incubated at 37ºC for 60min. Specific activity > 100 pmol/min/ug.
PubMed Link: B3GNT6 Human
Usage: For research only. Not for use in diagnostic or therapeutic procedures.
Country of Origin: USA