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Recombinant Human Core 3 synthase/B3GNT6 (β-1,3-N-Acetylglucosaminyltransferase 6), soluble fragment

B3GNT6

 

Description:

β­1,3­N­acetylglucosaminyltransferase 6 (β3GnT6) transfers N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to N-acetylgalactosamine (GalNAc).  β3GnT6 is also known as the core 3 synthase, because it creates the core 3 oligosaccharide structure in the mucin-type O-glycan, GlcNAcβ1­3GalNAcα1­serine/threonine (1).  Core 3 synthase is a type II transmembrane protein, and is localized to stomach, small intestine and colonic tissues (1).  It has long been known that synthesis of the core 3 structure is down-regulated in colonic cancer cells (2) and it has been shown that the expression of β3GnT6  is also down-regulated (1).  Colonic cancer cells transfected with β3GnT6 have reduced metastatic potential compared to non-transfected cells, and when injected into mice show decreased tumor formation  (3).  Pancreatic cancer cells transfected with β3GnT6 show a similar phenotype, which has been attributed to the addition of core3 O-glycans to α2β1 integrin (4).

  1. Iwai, T. et al. (2002) J. Biol. Chem. 277:12802.
  2. Vavasseur ,  F.  et al. (1995) Glycobiology 5:351.
  3. Iwai, T. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4572.
  4. Lee, S.H. et al. (2009) J. Biol. Chem. 284:17157.

Catalog #:  SBH-003- B3GNT6

Source :  Insect Cells, Accession # Q6ZMB0

Formulation:  Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 100 ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Purity:  Greater than 95% by SDS-PAGE

Reconstitution:  N/A

Biological Activity:  Measured by transfer of N-acetylglucosamine (GlcNAc)  from UDP-GlcNAc to 4-Nitrophenyl-N-acetylgalactosamine (4-NP-GalNAc, Sigma N4264), as measured with a phosphatase-coupled assay.  Coupling assay reactions contained:  25mM Tris pH 7.5, 150mM NaCl, 10mM MnCl2, 5mM CaCl2, 20% DMSO, 0.2mM UDP-GlcNAc, 0.6mM 4-NP-GalNAc, 1.67ug/mL ENTPD3/CD39L3, and 6ug/mL B3GnT6.  Incubated at 37ºC for 60min. Specific activity > 100 pmol/min/ug.

PubMed  Link: B3GNT6 Human

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA


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