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Mouse Anti-Human Bone Morphogenetic Proteins - 4
(Monoclonal Antibody)

Preparation:

Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant human BMP-4.

Purification:

This IgG2B antibody was purified by Protein G affinity chromatography.

Formulation:

Lyophilized from a 0.2 um filtered solution in 0.5X phosphate-buffered saline (PBS) at pH 7.2.

Catalog #:

SBH-ABMP4

Source:

Mouse

Stability:

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -800C.

Reconstitution:

When reconstituted in sterile water to a concentration of 0.5 mg/ml the antibody is stable for at least six weeks at 2-40C.

Applications

ELISA:

In a sandwich ELISA (assuming 100μl/well), a concentration of 1 μg/ml of this antibody will detect at least 0.5 ng/well of recombinant human BMP-4 when used with conjunction with goat anti-mouse horseradish peroxidase (Jackson Laboratories).

Western Blot:

To detect h-BMP-4 by Western Blot analysis this antibody can be used at a concentration of 2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant h-BMP-4 is 1.0 ng/lane.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (2 ng/ml), a concentration of 2 - 4 µg/ml of this antibody is required.

Country of Origin:

USA

Usage:

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.



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