Recombinant human Polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2), soluble fragment
Polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues (1). Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences (2). The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein (2). Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAca1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen (3). GalNAc-T2 is primarily located in Golgi, and is widely distributed in human tissues (1,4,5).
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Catalog #: SBH-003-GalNAc-T2
Source : Insect Cells, Accession # Q10471
Formulation: Sterile filtered solution in 20mM Hepes pH7 and 100mM NaCl, at a stock concentration of 360 ug/mL.
Stability: Stable for 4 weeks at 4C. Stable for 6 months at -80C. Avoid repeated freeze-thaw cycles.
Purity: Greater than 90% by SDS-PAGE.
Biological Activity: Measured by transfer of GalNAc from UDP-GalNAc to the peptide MEELGMAPALQPTQGAMPAF, enzyme reactions containing: 25mM Caco pH 7.4, 10mM MnCl, 0.25% Triton X100, 2mM UDP-GalNAc and 10mg (400ug/mL final) peptide. Incubated at 37ºC. Specific activity > 300 pmol/min/mg. Specific activity > 300 pmol/min/ug.
PubMed Link: GalNAc-T2 Human
Usage: For research only. Not for use in diagnostic or therapeutic procedures.
Country of Origin: USA