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Recombinant Human Interleukin-23

HIL-23 Applications

Description :

IL-23 is a proinflammatory heterodimeric protein composed of two subunits, a unique p19 subunit and a p40 subunit, which is shared with IL-12.IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ2. IL- 23 has been shown to enhance proliferation of memory T cells. It also stimulates the production of IFNgamma in NK cells, induces IL-17 production, and drives Th17 mediated responses. Recombinant IL-23 is a 53.5 kDa heterodimeric protein consisting of two subunits, p19 (170 amino acids) and p40 (306 amino acids).

Catalog #:

003-HIL23

Source :

High-5 (BTI-Tn-5B1-4) Insect Cells

Stability :

The lyophilized protein isstable for a few weeks at room temperature, but best stored at -200C. Reconstituted Human IL-23 should be stored in working aliquots at -200C.   

Purity :

Greater than 95% by SDS-PAGE.

Endotoxin Level:

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution:

We recommend a quick spin followed by reconstitution in 1x PBS pH 7.2-7.4 to a concentration of 0.1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or at -200C for future use.   

Biological Activity :

Determined by the stimulation of IFN-gamma production by PBMC.
 The ED50 for this effect is < 10 -20 ng/ml, corresponding to a specific activity of >1 x 105.

AA Sequence:

Human Interleukin-23 subunit alpha:

RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP

Human Interleukin -12 / 23 subunit beta (40KD):

IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

PubMed  Link :

IL-23

Usage:

For research only. Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute
for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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