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Recombinant Murine Interleukin-12 (p70)

Description :

Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+T cells and NK cells. Murine IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells. Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits.

Catalog #:

001-MIL12P70

Source :

CHO cells (Chinese Hamster Ovarian Cells)

Formulation :

Sterile filtered and then the protein was lyophilized in 1 x PBS pH 6.8.

Stability :

The lyophilized protein, is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted murine IL-12 should be stored in working aliquots at -200C.

Purity :

Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration . This solution can then be diluted into other aqueous buffers and stored at 40C for 3 months or below -100C for future use.

Biological Activity :

A. Determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-12. The expected ED50 for this effect is < 0.1 ng/ml, corresponding to a specific activity of >1 x107 units/mg.

B. Determined by the dose-dependent stimulation of the proliferation of Immature B Lymphocyte murine 2D6 cells.The expected ED50 for this effect is < 1.0 ng/ml.

PubMed  Link :

IL-12 Mouse

Usage:

For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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