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Mouse Anti-Human beta-NGF (Monoclonal Antibody)

Preparation:

Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human beta-NGF (human beta-Nerve Growth Factor).

Purification:

This IgG2b/Kappa antibody was purified by Protein G affinity chromatography.

Formulation:

Lyophilized from a 0.2 mm filtered solution in 0.5X phosphate-buffered saline (PBS) at pH 7.2.

Catalog #:

SBH-ABNGF

Source:

Mouse

Stability:

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -800C.

Reconstitution:

When reconstituted in sterile water to a concentration of 1 mg/ml the antibody is stable for at least six weeks at 2-40C.

Applications

ELISA:

In a sandwich ELISA (assuming 100μl/well), a concentration of 2 μg/ml of this antibody will detect at least 0.1 ng/well of recombinant human beta-NGF when used with conjunction with PeproTech’s biotinylated antigen-affinity purified anti-human ß-NGF (500-P85Bt) as the detection antibody at a concentration of at least 0.5 µg/ml.

Country of Origin:

USA

Usage:

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.



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