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Recombinant Human Sonic Hedgehog

Description :

Hedgehog (Hh) proteins are widely distributed throughout the animal kingdom and play important regulatory roles in various development processes. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93 % identical). Although, Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they signal through the same Patched (Ptc)/Smoothened (Smo) receptor complex, activate the same intracellular signaling pathway, and can substitute for each other in experimental systems. The mature biologically active form of Hh molecules is produced by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal half of the precursor molecule. Recombinant human Shh is a 20.6 kDa protein consisting of 174 amino acid residues, which correspond to the active N-terminal portion of the Shh precursor.

Catalog #:

003-SHH

Source :

High-5 (BTI-Tn-5B1-4) Insect Cells 

Formulation :

1X PBS, pH =7.0.

Stability :

The lyophilized protein, is stable for at least 2 years from date of receipt at -200C. Shh is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml

PubMed  Link :

Sonic Hedgehog

Usage:

For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.  

Country of Origin :

USA



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