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Recombinant Human Soluble IL-2 Receptor alpha (s-IL-2R-a)

Description :

Human IL-2 exerts its biological effects via signaling through its receptor system, IL-2R. IL-2 and its receptor (IL-2R) are required for T-cell proliferation and other fundamental functions which are crucial of the immune response. IL-2R consists of 3 noncovalently linked type I transmembrane proteins which are the alpha (p55), beta (p75), and gamma (p65) chains. The IL-2R alpha chain contains an extracellular domain of 219 amino acids, a transmembrane domain of 19 amino acids, and an intracellular domain of 13 amino acids. The secreted extracellular domain of IL-2R alpha (s-IL-2R-a), also called TAC-antigen, is expressed on leukemia cells, lymphoma cells, approximately 10% NK cells, as well as recently activated T and B cells. Recombinant human s-IL-2R-a is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and Mass Spectrometry.

Catalog #:

003-SIL2Ra

Source :

High-5 (BTI-Tn-5B1-4) Insect Cells

Formulation :

The sterile filtered solution was lyophilized from 1x PBS.

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted s-IL-2R alpha should be stored in working aliquots at 40C.

Purity :

Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or -200C for future use.

Biological Activity :

Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5 - 1.5 ng/ml.

PubMed  Link :

IL-2 receptor-alpha

Usage:

For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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