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Recombinant Human Soluble IL-4 Receptor alpha (s-IL-4R-a)

Description :

Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4R alpha is a 23.7 kDa protein containing 207 amino acid residues consisting of only the extracellular domain of the IL-4R alpha.

Catalog #:

002-SIL4Ra

Source :

HEK 293 cells

Formulation :

The sterile filtered solution was lyophilized from 1x PBS, pH 7.0.

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted IL-4R alpha should be stored in working aliquots at -200C.

Purity :

Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or -200C for future use.

Biological Activity :

The ED50 as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is [5.0 ng/ml (in the presence of 0.5 ng/ml IL-4), corresponding to a specific activity of ] 2 x 10 exp5 units/mg.

AA Sequence :

MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA QCYNTTWSEW SPSTKWHNSY REPFEQH

PubMed  Link :

IL-4 receptor-alpha

Usage:

For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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