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Recombinant Human Soluble IL-6 Receptor (s-IL-6R)

s-IL-6R Applications

Description :

The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. SBH Sciences' recombinant human sIL-6R alpha is a 37.6 kDa protein, consisting of the extra cellular domain of the sIL-6R alpha chain (338 amino acids).

Catalog #:

002-SIL6R

Source :

HEK 293 cells

Formulation :

The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2.

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted sIL-6R should be stored in working aliquots at -200C.

Purity :

Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or stored at -200C for futures use.

Biological Activity :

The ED50 as determined by it's ability to intensify the IL-6 induced growth inhibition of murine M1 cells is < 10.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.

AA Sequence :

LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH ASGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VCEWGPRSTP SLTTKAVLLV RKFQNSPAED FQEPCQYSQE SQKFSCQLAV PEGDSSFYIV SMCVASSVGS KFSKTQTFQG CGILQPAPPA NITVTAVARN PRWLSVTWQD PHSWNSSFYR LRFELRYRAE RSKTFTTWMV KDLQHHCVIH DAWSGLRHVV QLRAQEEFGQ GEWSEWSPEA MGTPWTESRS PPAENEVSTP MQALTTNKDD DNILFRDSAN ATSLPVQD

PubMed  Link :

IL-6 receptor-alpha

Usage:

For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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