Recombinant Human ST3Gal1 (CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1), soluble fragment
ST3Gal1 catalyzes transfer of the sialic acid Neu5Ac from CMP-Neu5Ac (cytidine 5'-monophosphono-N-acetylneuraminic acid) to Galβ1,3GalNAc on glycoproteins or glycolipids in an α2,3 linkage (1). It is a type II transmembrane protein that is normally localized to the golgi, but can be cleaved to yield a soluble product. Sialylation of the core-1 structure (Galβ1,3GalNAc-O-Ser/Thr) by ST3Gal1 prevents formation of the core-2 structure (GlcNAcβ1,6(Galβ1,3)GalNAc-Ser/Thr). On CD8+ T-cells this prevents induction of either apoptosis or differentiation into memory cells (2). Overexpression of ST3Gal1 in breast cancer cells correlated with the presence of the core-1 based marker SM3, whereas healthy cells displayed a higher ratio of core-2 based epitopes (3). Overexpression of ST3Gal1 in colorectal carcinoma was found to be related to poor patient survival (4).
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Catalog #: SBH-003- ST3Gal1
Source : Insect Cells, Accession # Q11201
Formulation: Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 100 ug/mL.
Stability: Stable for 4 weeks at 4C. Stable for 6 months at -80C. Avoid repeated freeze-thaw cycles.
Purity: Greater than 90% by SDS-PAGE.
Biological Activity: In progress.
PubMed Link: ST3Gal1 Human
Usage: For research only. Not for use in diagnostic or therapeutic procedures.
Country of Origin: USA