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Recombinant Human Transforming Growth Factor-beta 1

Description :

Transforming Growth Factor-beta1 (TGF-beta1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-beta1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues.

Catalog #:

001-TGF1

Source :

CHO Cells

Formulation :

Lyophilized from Sodium Citrate, pH 3.5.

Stability :

The lyophilized TGF-beta1 is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted TGF-beta1 should be stored at -200C.

Purity :

Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in water to a concentration of 50 ug/ml. This solution can then be stored at 40C for 1 month. It is recommended that further dilutions be made in PBS containing 2mg/ml albumin and stored at -800C.

Biological Activity :

The ED50 was determined by TGF-beta1 ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of 2 x 107 units/mg.

AA Sequence :

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

PubMed  Link :

TGFbeta1

Usage:

Made using a license from PHS, NIH. Patent Pending. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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