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Recombinant Human Tumor Necrosis Factor-beta

Description :

Tumor Necrosis Factor-beta (TNF-beta ; also known as: Lymphotoxin-alpha) is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-beta is an 18.6 kDa protein containing 172 amino acid residues. Due to glycosylation, TNF-beta has an approximate molecular weight of 19.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

Catalog #:

003-TNFb

Source :

High-5 (BTI-Tn-5B1-4) Insect Cells

Formulation :

The sterile filtered solution was lyophilized from 10mM Sodium Phosphate, pH 7.0 + 50mM NaCl.

Stability :

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted TNF-beta should be stored in working aliquots at -200C.

Purity :

Greater than 95% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or -200C for future use.

Biological Activity :

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.

PubMed  Link :

TNFbeta+AND+human

Usage:

For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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