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Recombinant Human Hepatocyte Growth Factor (h-HGF)

HGF Applications

Description :

Human Hepatocyte Growth Factor is also known as Scatter Factor and Hepatopoeitin A. HGF is a potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. Recombinant human HGF is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (234 amino acids).

Catalog #:

003-HGF

Source :

High-5 (BTI-Tn-5B1-4) Insect Cells

Formulation :

The sterile filtered solution was lyophilized from 0.1M L-Arganine HCl + 10 mM Tris, pH 8.0.

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted HGF should be stored in working aliquots at -200C.

Purity :

Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in water to a concentration of 0.5 mg/ml. This solution can then be diluted into other buffers and stored at 40C for 1 week or stored at -200C for future use.

Biological Activity :

The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

PubMed  Link :

HGF Hepatocyte

Usage:

For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA



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