Preparation: |
Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse
myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived,
recombinant human BAFF.
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Purification: |
This IgG1 antibody was purified by Protein G affinity chromatography.
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Formulation: |
Lyophilized from a 0.2 mm filtered solution in 0.5X phosphate-buffered saline (PBS) at pH 7.2.
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Catalog #: |
SBH-ABAFF
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Source: |
Mouse
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Stability: |
The lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -800C.
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Reconstitution: |
When reconstituted in sterile water to a concentration of 1 mg/ml the antibody is stable for at
least six weeks at 2-40C.
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Applications |
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ELISA: |
In a sandwich ELISA (assuming 100μl/well), a concentration of 5 μg/ml of this antibody
will detect at least 0.5 ng/well of recombinant human BAFF when used with conjunction with
goat anti-mouse horseradish peroxidase (Jackson Laboratories).
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Country of Origin: |
USA
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Usage: |
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
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