Preparation: |
Monoclonal antibodies were produced from a
hybridoma resulting from the fusion of a mouse myeloma with B cells
obtained from a mouse immunized with purified, E. coli-derived,
recombinant human BMP-7.
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Purification: |
This IgG1 antibody was purified by Protein
G affinity chromatography.
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Formulation: |
Lyophilized from a 0.2 um filtered solution
in 0.5X phosphate-buffered saline (PBS) at pH 7.2.
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Catalog #: |
SBH-ABMP7
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Source: |
Mouse
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Stability: |
The lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -800C.
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Reconstitution: |
When reconstituted in sterile water to a concentration
of 0.5 mg/ml the antibody is stable for at least six weeks at 2-40C.
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Applications |
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ELISA: |
In a sandwich ELISA (assuming 100μl/well),
a concentration of 1 μg/ml of this antibody will detect at least
0.5 ng/well of recombinant human BMP-7 when used with conjunction
with goat anti-mouse horseradish peroxidase (Jackson Laboratories).
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Western Blot: |
To detect h-BMP-7 by Western Blot analysis
this antibody can be used at a concentration of 2 µg/ml. Used
in conjunction with compatible secondary reagents the detection
limit for recombinant h-BMP-7 is 2.5 ng/lane.
|
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Biological Activity:
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To yield one-half maximal stimulation [ED50]
of the biological activity of hBMP-7 (50 ng/ml), a concentration
of 0.4 - 0.6 µg/ml of this antibody is required.
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Country of Origin: |
USA
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Usage: |
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
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