Preparation: |
Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse
myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived,
recombinant human Eotaxin-3.
|
Purification: |
This IgG1 antibody was purified by Protein G affinity chromatography.
|
Formulation: |
Lyophilized from a 0.2 mm filtered solution in 0.5X phosphate-buffered saline (PBS) at pH 7.2.
|
Catalog #: |
SBH-AEOTAXIN3.
|
Source: |
Mouse
|
Stability: |
The lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -800C.
|
Reconstitution: |
When reconstituted in sterile water to a concentration of 1 mg/ml the antibody is stable for at
least six weeks at 2-40C.
|
Applications |
|
ELISA: |
This antibody can be used at a concentration of 1-2 μg/ml, as a capture antibody in conjunction
with a compatible secondary reagent to yield satisfactory results in a sandwich ELISA.
|
Neutralization: |
To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2 μg/ml), a concentration of 1-5 μg/ml of this antibody is required.
|
Country of Origin: |
USA
|
Usage: |
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
|
