Description : |
Granzyme B is a cysteine protease found
in the cytoplasmic granules of cytolytic T lymphocytes (CTL)
and natural killer (NK) cells. Granzyme B is required for
the induction of target cell lysis, which occurs as part of
cell mediated immune responses, and can activate apoptosis
in target cells by both caspase dependent and caspase independent
mechanisms. Proteolytic cleavage of substrates by Granzyme
B takes place primarily after aspartic acid residues. Recombinant
murine Granzyme B is a glycosylated 227 amino acid protein,
comprising the mature active portion of the murine Granzyme
B precursor. The apparent molecular weight is 28.9 kDa by
mass spectrometry
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Catalog #: |
003-mGranzyme B
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Source : |
High-5 (BTI-Tn-5B1-4) Insect Cells |
Formulation : |
Lyophilized from 1X PBS |
Stability : |
The lyophilized Granzyme B is stable
for a few weeks at room temperature, but best stored at -200C.
Reconstituted Granzyme B should be stored at -200C.
|
Purity : |
Greater than 98% by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
|
Reconstitution : |
We recommend a quick spin followed by
reconstitution in water to a concentration of 50 ug/ml. This
solution can then be stored at 40C
for 1 month. It is recommended that further dilutions be made
in PBS containing 2mg/ml albumin and stored at -800C.
|
Biological Activity : |
Determined by its ability to cleave a
synthetic chromogenic Granzyme B substrate. The expected specific
activity, when using the Ac-IEPD-pNA substrate at 250C, is
greater than 750 nM/min per μg of enzyme.
|
PubMed Link : |
Granzyme B |
Usage: |
For research Purposes Only - Made using
a cell BTI-Tn-5B1-4 under license from the Boyce Thompson
Institute for Plant Research, Inc. Not for use in diagnostic
or therapeutic procedures.
|
Country of Origin : |
USA |
