Description : |
Human IL-4 exerts its biological effects via signaling through its receptor
system, IL-4R. There are two types of the IL-4 receptor complexes, the type I
receptor consisting of the IL-4R alpha and the common gamma chain, and the
type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R
alpha chain contains an extracellular domain of 207 amino acids, a
transmembrane domain of 24 amino acids, and an intracellular domain of 569
amino acids. The secreted extracellular domain, sIL-4R alpha, also called
CD124 is capable of blocking IL-4 activities. This receptor plays a major
role in the regulation of the differentiation of naive CD4+ T cells and class
switching to IgG1 and IgE. Recombinant human sIL-4R alpha is a 23.7 kDa
protein containing 207 amino acid residues consisting of only the
extracellular domain of the IL-4R alpha. |
Catalog #: |
002-SIL4Ra |
Source : |
HEK 293 cells |
Formulation : |
The sterile filtered solution was lyophilized from 1x PBS, pH 7.0. |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature, but
best stored at -200C. Reconstituted IL-4R alpha should be stored in working
aliquots at -200C. |
Purity : |
Greater than 97% by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
|
Reconstitution : |
We recommend a quick spin followed by reconstitution in water to a
concentration of 0.1-1.0mg/ml. This solution can then be diluted into other
aqueous buffers and stored at 40C for 1 week or -200C for future use. |
Biological Activity : |
The ED50 as determined by its ability to inhibit the IL-4 dependent
proliferation of human TF-1 cells is [5.0 ng/ml (in the presence of 0.5
ng/ml IL-4), corresponding to a specific activity of ] 2 x 10 exp5 units/mg. |
AA Sequence : |
MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA QCYNTTWSEW SPSTKWHNSY REPFEQH
|
PubMed Link : |
IL-4 receptor-alpha |
Usage: |
For research only. Not for use in diagnostic or therapeutic procedures. |
Country of Origin : |
USA |
