Description
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. SBH Sciences’ recombinant human sIL-6R alpha is a 37.6 kDa protein, consisting of the extra cellular domain of the sIL-6R alpha chain (338 amino acids).
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml.
Storage: This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or stored at -20°C for futures use..
For research Purposes Only. Not for use in diagnostic or therapeutic procedures.
| PROPERTIES | |
|---|---|
| Species | Human |
| Expression System |
Human Embryonic Stem Cells, HEK-293 |
| Molecular Weight | 37.6 kDa |
| Full Length | 338 amino acids |
| Purity |
≥98% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity | The ED50 as determined by its ability to intensify the IL-6 induced growth inhibition of murine M1 cells is < 10.0 ng/ml, in the presence of 20 ng/ml of rhIL-6 |
| Storage | -20°C |
