Applications:
ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 1 μg/ml of this antibody will detect at least 0.5 ng/well of recombinant human BMP-4 when used with conjunction with goat anti-mouse horseradish peroxidase (Jackson Laboratories).
Western Blot: To detect h-BMP-4 by Western Blot analysis this antibody can be used at a concentration of 2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant h-BMP-4 is 1.0 ng/lane.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (2 ng/ml), a concentration of 2 – 4 µg/ml of this antibody is required.
Reconstitution: When reconstituted in sterile water to a concentration of 0.5 mg/ml the antibody is stable for at least six weeks at 2-40C.
For research purposes only. Not for use in diagnostic or therapeutic procedures.
| PROPERTIES | |
|---|---|
| Species | Mouse |
| Purification | This IgG2B antibody was purified by Protein G affinity chromatography |
|
Stability |
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C |
| Preparation | Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant human BMP-4 |
| Form |
Lyophilized |
| Storage |
20°C |
