Utilization of Differentiated THP-1 Cell Line for Drug Discovery

The THP-1 cell line, derived from human leukemia monocytic cells, is widely used in drug discovery research to explore mechanisms, signaling pathways, drug transport, and immune responses. This in-vitro model uniquely differentiates into macrophage-like cells when treated with specific agents such as phorbol 12-myristate 13-acetate (PMA). This makes it ideal for studying macrophage functions and their responses to various stimuli in a controlled environment.

THP-1 cells serve as an excellent model for investigating various biological processes, including immune responses, inflammation, pathogen interactions, and drug effects on macrophages. The cell line is accessible, scalable, and well-characterized, making it ideal for studying monocyte differentiation and macrophage behavior.

Macrophage Polarization

Macrophages are critical white blood cells involved in immunity, tissue repair, and inflammation regulation. These cells can be polarized into two distinct phenotypes—M1 (pro-inflammatory) and M2 (anti-inflammatory)—each with unique roles in the immune response.

M1 Macrophages (Pro-Inflammatory):

Induced by IFN-γ and lipopolysaccharide (LPS), M1 macrophages are vital for fighting infections and tumors. They release cytokines like TNF-α, IL-1β, and IL-6, contributing to inflammation. However, excessive M1 activation can lead to tissue damage in chronic diseases.

M2 Macrophages (Anti-inflammatory):

Induced by IL-4 and IL-13, M2 macrophages help resolve inflammation and promote tissue repair. However, they can also be involved in tumor progression, angiogenesis, metastasis, and resistance to therapies, making them a complex target in disease treatment.

Understanding the balance between M1 and M2 macrophages is crucial for developing treatments for conditions driven by excessive inflammation (such as autoimmune diseases) or impaired resolution of inflammation (like cancer).

Our Cell Culture Services include:

• THP-1 Cell Differentiation: We offer differentiation of THP-1 cells into macrophages and their subsequent polarization into M1 and M2 subtypes, enabling researchers to study macrophage behavior and immune responses.
  
  
• Cytokine and Antibody Production: We supply recombinant cytokines and monoclonal antibodies for applications in preclinical research and therapeutic development.
  
  
• Custom Cell-Based Assays: Our assays are designed to screen for biological or cytotoxic activity, tailored to meet your specific needs.
  
  
• Protein Purification & Production: We specialize in protein purification, scale-up, and mid-scale production to ensure high-quality yields for your projects.

Let us help you:

• Streamline your production process.
• Reduce your development time.
• Increase your yields while maintaining exceptional purity.
• Reduce production costs.

SBH Sciences welcomes inquiries from those seeking a dependable partner and collaborator to develop pharmaceutical and biotechnology-derived products. Contact us to discuss how we can help accelerate your research with customized THP-1 cell models and macrophage differentiation services.