Description : |
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23, or can form a homodimer designated as IL-12 p80. Elevated levels of IL-12 p80 are correlated with macrophage recruitment and increased inflammation in asthma and respiratory viral infection models. Recombinant human IL-12 p80 is an 80.0 kDa disulfide linked homodimer consisting of two p40 chains of human IL-12. |
Catalog #: |
003-HIL12P80 |
Source : |
Insect cells |
Formulation : |
Sterile filtered and then the protein was lyophilized in 1 x PBS pH 6.8. |
Stability : |
The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted IL-12 p80 is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles. |
Purity : |
Greater than 95% by SDS-PAGE. |
Reconstitution : |
We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration .
This solution can then be diluted into other aqueous buffers and stored at 40C for 3 months or below -100C for future use.
|
Endotoxin Level: |
Endotoxin level
is less than 0.1 ng per μg (1EU/μg). |
Biological Activity : |
Not available. |
AA Sequence: |
IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
PubMed Link : |
IL-12 p80 |
Usage: |
For research only. Not for use in diagnostic or therapeutic procedures. |
Country of Origin : |
USA |
