--- title: Molecular Biology Services description: Comprehensive molecular biology services, isolation of DNA and RNA, real time PCR, gene knockdown, transfection, molecular diagnostic assay development --- [![SBH Sciences](https://sbhsciences.com/hs-fs/hubfs/Imported%20sitepage%20images/sbhlogo.png?width=155&height=74&name=sbhlogo.png "SBH Sciences")](https://sbhsciences.com/) - Open submenu for ABOUT US - [ABOUT SBH](https://sbhsciences.com/about-sbh) - [LEADERSHIP](https://sbhsciences.com/sbh-leadership) - [CONTACT](https://sbhsciences.com/contact) - [CAREERS](https://sbhsciences.com/careers) - [SBH BIOVENTURE](https://sbhsciences.com/sbh-bioventure) - [SOLUTIONS](https://sbhsciences.com/services) Show submenu for SOLUTIONS - [CELL BASED ASSAYS](https://sbhsciences.com/cell-based-assay-services) - Open submenu for PRODUCT SERVICES - [PROTEINS](https://sbhsciences.com/protein-production-services) - [MOLECULAR BIOLOGY](https://sbhsciences.com/molecular-biology-services) - [CELL CULTURE](https://sbhsciences.com/cell-culture-services) - 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[ORGANOIDS](https://sbhsciences.com/blog/organoids-mini-organs-grown-for-research-and-drug-development) - [EXOSOMES](https://sbhsciences.com/exosome-services) - [BIOMARKER TESTING](https://sbhsciences.com/biomarker-testing) - Open submenu for DRUG DISCOVERY - [ADCC](https://sbhsciences.com/antibody-development-1) - [ELISA](https://sbhsciences.com/elisa-assays) - [BIOANALYTICAL](https://sbhsciences.com/bioanalytical-discovery-services) - [BIOSIMILARS](https://sbhsciences.com/biosimilars) - [CELL & GENE THERAPY](https://sbhsciences.com/cgt) - [SMALL MOLECULE](https://sbhsciences.com/small-molecule) - [FTE MODEL](https://sbhsciences.com/fte-model) - [CONSULTING](https://sbhsciences.com/mentoring) - Open submenu for INDICATIONS - [NEUROLOGY](https://sbhsciences.com/neurology) - [METABOLIC](https://sbhsciences.com/metabolic) - [ONCOLOGY](https://sbhsciences.com/oncology) - [KIDNEY](https://sbhsciences.com/kidney) - [MUSCLE](https://sbhsciences.com/muscle) - Open submenu for PRODUCTS - [CYTOKINES](https://sbhsciences.com/cytokines) - [MONOCLONAL ANTIBODIES](https://sbhsciences.com/monoclonal-antibodies) - [GLYCOBIOLOGY](https://sbhsciences.com/glycobiology) - Open submenu for INSIGHTS - [NEWS AND BLOG](https://sbhsciences.com/sbh-blog-and-news) - [REFERENCES AND PUBLICATIONS](https://sbhsciences.com/references) - [STRATEGIC PARTNERSHIPS](https://sbhsciences.com/partnerships) Search Search Search Search # Cell and Molecular Biology Services ![grid\_2021\_bioservices-1](https://sbhsciences.com/hs-fs/hubfs/grid_2021_bioservices-1.jpg?width=300&height=250&name=grid_2021_bioservices-1.jpg)SBH Sciences offers comprehensive molecular biology services, including: **Isolation of DNA and RNA** - Extraction and purification of RNA and genomic DNA from cultured cells and a variety of tissues - Assessment of concentration and purity by Nanodrop spectrophotometry and gel electrophoresis **Real-Time PCR: qPCR, RT-qPCR, dPCR** - Multiple platforms, including the Applied Biosystems StepOnePlusTM, QuantStudioTM 6 Pro, and QuantStudioTM Absolute Q Digital PCR System. - Gene expression analysis using TaqMan and SYBR Green detection chemistries. - RNA extraction, cDNA synthesis, primer design and validation, assay optimization - Analysis of target gene expression using DDCt methodology - Assays for gene therapy applications - vector copy number, biodistribution, AAV titer Oligonucleotide Bioanalysis: siRNA quantification[![Screenshot 2026-08-11 at 2.37.23 PM](https://sbhsciences.com/hs-fs/hubfs/Screenshot%202026-08-11%20at%202.37.23%20PM.png?width=261&height=337&name=Screenshot%202026-08-11%20at%202.37.23%20PM.png)](https://sbhsciences.com/hubfs/SBH_HIT_qRT-PCR_Capability_Brochure_Red_Black_White.pdf) SBH Sciences uses heating-in-Triton quantitative reverse transcription PCR (HIT qRT-PCR) to directly quantify administered siRNA in tissue and plasma, providing a sensitive measure of oligonucleotide exposure, distribution, and persistence in vivo. The method efficiently releases siRNA from formulations and protein-bound matrices, then uses controlled heating and sequence-specific stem-loop reverse transcription to minimize reannealing and accurately quantify the selected sense or antisense strand. By analyzing plasma or tissue lysates directly without conventional RNA purification, HIT qRT-PCR reduces sample processing, turnaround time, and cost while maintaining high sensitivity and a broad quantitative range. This approach can help researchers determine where an siRNA drug distributes, how long it remains detectable, and how tissue or plasma exposure relates to downstream target engagement. [Download our Whitepaper: Sensitive, Rapid and Cost-Effective Quantification of siRNA in Tissue and Plasma](https://sbhsciences.com/hubfs/SBH_HIT_qRT-PCR_Capability_Brochure_Red_Black_White.pdf)   **Transfection** - Transfection of mammalian cell lines with plasmid DNA, mRNA, or siRNA. - Optimization of cell growth and transfection conditions - Analysis of gene expression/knockdown by multiple methods, including fluorescence microscopy, flow cytometry, ELISA, Western blot, and qPCR **Gene Knockdown** - Stable knockdown of target gene expression via transduction of lentiviral shRNA. - Puromycin titration to establish a kill curve for new cell lines - Design and cloning of shRNA oligos containing the target sequence(s) - Lentivirus production and transduction of cells at optimal MOI - Selection of puromycin-resistant populations and confirmation of knockdown by qPCR or Western blot. **Molecular Diagnostic Assays** - Development and application of PCR-based assays for infectious diseases. - RT-qPCR, dPCR or isothermal amplification (LAMP)-based assays - Optimization of sample preparation - Primer design and validation - Sample testing. ![Gene\_knockdown](https://sbhsciences.com/hs-fs/hubfs/Imported%20sitepage%20images/Gene_knockdown.jpg?width=554&height=307&name=Gene_knockdown.jpg)   Please [contact us](https://sbhsciences.com/contact) with your specific needs or any questions related to Cell and Molecular Biology services. 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