Description
Activin-A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin-A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin-A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin. Human Activin-A is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues.
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 50 μg/ml (e.g., 5 μg/100μl) will yield a solution of 10 mM Citric Acid, pH 3.0.
Storage: This solution can then be stored at room temperature or at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20°C.
| PROPERTIES | |
|---|---|
| Species | Human, Rat, Mouse |
| Expression System | High-5, Insect Cells |
| Molecular Weight | 26 kDa |
| Full Length | 118 amino acids |
| Purity |
≥95% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity | The ED50 was determined by its ability to inhibit the murine MPC-11 cells. The expected ED50 is 0.5 - 2.0 ng/ml |
| Storage | -20°C |