--- title: "High Throughput Screening: AlphaLISA (Part I)" description: Highlighting AlphaLISA - a powerful alternative with enhanced sensitivity, no wash steps, and scalability for high-throughput screening. image: https://sbhsciences.com/hubfs/blog_sbh_author.jpg --- [![SBH Sciences](https://sbhsciences.com/hs-fs/hubfs/Imported%20sitepage%20images/sbhlogo.png?width=155&height=74&name=sbhlogo.png "SBH Sciences")](https://sbhsciences.com/) - Open submenu for ABOUT US - [ABOUT SBH](https://sbhsciences.com/about-sbh) - [LEADERSHIP](https://sbhsciences.com/sbh-leadership) - [CONTACT](https://sbhsciences.com/contact) - [CAREERS](https://sbhsciences.com/careers) - [SBH BIOVENTURE](https://sbhsciences.com/sbh-bioventure) - [SOLUTIONS](https://sbhsciences.com/services) Show submenu for SOLUTIONS - [CELL BASED ASSAYS](https://sbhsciences.com/cell-based-assay-services) - Open submenu for PRODUCT SERVICES - [PROTEINS](https://sbhsciences.com/protein-production-services) - [MOLECULAR BIOLOGY](https://sbhsciences.com/molecular-biology-services) - [CELL CULTURE](https://sbhsciences.com/cell-culture-services) - [ORGANOIDS](https://sbhsciences.com/blog/organoids-mini-organs-grown-for-research-and-drug-development) - [EXOSOMES](https://sbhsciences.com/exosome-services) - [BIOMARKER TESTING](https://sbhsciences.com/biomarker-testing) - Open submenu for DRUG DISCOVERY - [ADCC](https://sbhsciences.com/antibody-development-1) - [ELISA](https://sbhsciences.com/elisa-assays) - [BIOANALYTICAL](https://sbhsciences.com/bioanalytical-discovery-services) - [BIOSIMILARS](https://sbhsciences.com/biosimilars) - [CELL & GENE THERAPY](https://sbhsciences.com/cgt) - [SMALL MOLECULE](https://sbhsciences.com/small-molecule) - [FTE MODEL](https://sbhsciences.com/fte-model) - [CONSULTING](https://sbhsciences.com/mentoring) - Open submenu for INDICATIONS - [NEUROLOGY](https://sbhsciences.com/neurology) - [METABOLIC](https://sbhsciences.com/metabolic) - [ONCOLOGY](https://sbhsciences.com/oncology) - [KIDNEY](https://sbhsciences.com/kidney) - [MUSCLE](https://sbhsciences.com/muscle) - Open submenu for PRODUCTS - [CYTOKINES](https://sbhsciences.com/cytokines) - [MONOCLONAL ANTIBODIES](https://sbhsciences.com/monoclonal-antibodies) - [GLYCOBIOLOGY](https://sbhsciences.com/glycobiology) - Open submenu for INSIGHTS - [NEWS AND BLOG](https://sbhsciences.com/sbh-blog-and-news) - [REFERENCES AND PUBLICATIONS](https://sbhsciences.com/references) - [STRATEGIC PARTNERSHIPS](https://sbhsciences.com/partnerships) - Open submenu for ABOUT US - [ABOUT SBH](https://sbhsciences.com/about-sbh) - [LEADERSHIP](https://sbhsciences.com/sbh-leadership) - [CONTACT](https://sbhsciences.com/contact) - [CAREERS](https://sbhsciences.com/careers) - [SBH BIOVENTURE](https://sbhsciences.com/sbh-bioventure) - [SOLUTIONS](https://sbhsciences.com/services) Show submenu for SOLUTIONS - [CELL BASED ASSAYS](https://sbhsciences.com/cell-based-assay-services) - Open submenu for PRODUCT SERVICES - [PROTEINS](https://sbhsciences.com/protein-production-services) - [MOLECULAR BIOLOGY](https://sbhsciences.com/molecular-biology-services) - [CELL CULTURE](https://sbhsciences.com/cell-culture-services) - [ORGANOIDS](https://sbhsciences.com/blog/organoids-mini-organs-grown-for-research-and-drug-development) - [EXOSOMES](https://sbhsciences.com/exosome-services) - [BIOMARKER TESTING](https://sbhsciences.com/biomarker-testing) - Open submenu for DRUG DISCOVERY - [ADCC](https://sbhsciences.com/antibody-development-1) - [ELISA](https://sbhsciences.com/elisa-assays) - [BIOANALYTICAL](https://sbhsciences.com/bioanalytical-discovery-services) - [BIOSIMILARS](https://sbhsciences.com/biosimilars) - [CELL & GENE THERAPY](https://sbhsciences.com/cgt) - [SMALL MOLECULE](https://sbhsciences.com/small-molecule) - [FTE MODEL](https://sbhsciences.com/fte-model) - [CONSULTING](https://sbhsciences.com/mentoring) - Open submenu for INDICATIONS - [NEUROLOGY](https://sbhsciences.com/neurology) - [METABOLIC](https://sbhsciences.com/metabolic) - [ONCOLOGY](https://sbhsciences.com/oncology) - [KIDNEY](https://sbhsciences.com/kidney) - [MUSCLE](https://sbhsciences.com/muscle) - Open submenu for PRODUCTS - [CYTOKINES](https://sbhsciences.com/cytokines) - [MONOCLONAL ANTIBODIES](https://sbhsciences.com/monoclonal-antibodies) - [GLYCOBIOLOGY](https://sbhsciences.com/glycobiology) - Open submenu for INSIGHTS - [NEWS AND BLOG](https://sbhsciences.com/sbh-blog-and-news) - [REFERENCES AND PUBLICATIONS](https://sbhsciences.com/references) - [STRATEGIC PARTNERSHIPS](https://sbhsciences.com/partnerships) Search Search Search Search ![High Throughput Screening: AlphaLISA (Part I)](https://sbhsciences.com/hubfs/blog_sbh_author.jpg) # *Mar 25, 2025 |* High Throughput Screening: AlphaLISA (Part I) Highlighting AlphaLISA - a powerful alternative with enhanced sensitivity, no wash steps, and scalability for high-throughput screening. ## *Share* - [mailto:?&subject=High%20Throughput%20Screening:%20AlphaLISA%20(Part%20I)&body=High%20Throughput%20Screening:%20AlphaLISA%20(Part%20I)%0A(https%3A%2F%2Fsbhsciences.com%2Fblog%2Fhigh-throughput-screening-alphalisa)](mailto:?&subject=High%20Throughput%20Screening:%20AlphaLISA%20(Part%20I)&body=High%20Throughput%20Screening:%20AlphaLISA%20(Part%20I)%0A(https%3A%2F%2Fsbhsciences.com%2Fblog%2Fhigh-throughput-screening-alphalisa)) - - - Following our recent discussion on [ELISA](https://sbhsciences.com/blog/why-elisa-remains-essential-in-rd) we’re now highlighting AlphaLISA - a powerful alternative with enhanced sensitivity, no wash steps, and scalability for high-throughput screening. **What is AlphaLISA?** AlphaLISA (Amplified Luminescent Proximity Homogeneous Assay-Linked Immunosorbent Assay) is a bead-based assay technology used for the detection and quantitation of biomolecules such as proteins, peptides, and small molecules; all in a highly sensitive, specific, and time efficient manner. This technique is particularly well suited for applications in the biotech industry including drug discovery, biomarker research, and life sciences due to its robustness, flexibility, and scalability. **How Does AlphaLISA Work?** AlphaLISA relies on the proximity-based interaction between donor and acceptor beads. When these beads are brought close together by a specific interaction between an analyte and the biomolecules conjugated to the beads, excitation of the donor beads by a laser or LED light sources causes the release of singlet oxygen molecules. These singlet oxygen molecules can diffuse a short distance, and if they reach the acceptor beads, trigger a chemiluminescent reaction that results in a bright fluorescent signal. The intensity of this signal is directly proportional to the concentration of the target analyte in the sample. Simply, AlphaLISA is a streamlined workflow that allows the detection and quantification of both simple and complex sample types. ![AlphaLISA](https://sbhsciences.com/hs-fs/hubfs/AlphaLISA1.jpg?width=735&height=435&name=AlphaLISA1.jpg) A biotinylated antibody binds the streptavidin-coated donor beads, while a second antibody is conjugated to the AlphaLISA acceptor beads. In the presence of the analyte, the beads come together, and excitation at 680 nm generates singlet oxygen, triggering light emission at 615 nm. **Components** - **Donor Beads:** Contain a photosensitizer that upon excitation produces singlet oxygen. - **Acceptor Beads:** Contain thioxene derivatives that emit light upon interaction with singlet oxygen. - **Capture and Detection Reagents:** These are typically antibodies or other molecules with high specificity for the target analyte; and are conjugated to the donor and acceptor beads. **AlphaLISA Advantages** - **High Sensitivity:** The technology is capable of detecting very low levels of analytes, making it suitable for applications where the target molecule is present at low concentrations. - **Reduces Background Signal:** The requirement for proximity between the donor and acceptor beads for signal generation minimizes background noise, leading to high signal to noise ratios. - **Flexibility and Versatility:** AlphaLISA assays can be designed for a wide range of targets, including proteins, peptides, small molecules, and even cellular targets. - **No Wash Steps:** Unlike traditional ELISA, AlphaLISA is a homogeneous assay that doesn’t require separation or wash steps, simplifying the workflow and reducing the total assay time. - **Scalability:** The assay can be easily scaled from small to high throughout formats, accommodating the needs of both research and development and large-scale screening applications.   **Standard Curve For IL-6** **![AlphaLISA chart](https://sbhsciences.com/hs-fs/hubfs/AlphaLISA2.jpg?width=720&height=335&name=AlphaLISA2.jpg)** The graph above shows a representative standard curve for IL-6 generated using an AlphaLISA immunoassay. This data highlights the assay's sensitivity, with a lower detection limit (LDL) of 1.3 pg/mL, and its strong correlation (r² = 0.9948) in the linear range. **Partnering For Success with AlphaLISA** AlphaLISA is a powerful tool for the biotech industry, offering high sensitivity, specificity, and throughput capabilities for the quantitative analysis of biomolecules. Its adaptability across a range of applications from drug discovery to biomarker research makes it an invaluable asset in the biotech toolkit. SBH Sciences can perform any of the dozens of developed AlphaLISA assays to support your research and development needs. Please [contact SBH Sciences](https://sbhsciences.com/contact) to discuss how our AlphaLISA expertise can accelerate your research.   Sources: [https://www.cisbio.eu/content/alphalisa-technology-basics/](https://www.cisbio.eu/content/alphalisa-technology-basics/) [https://www.revvity.com/](https://www.revvity.com/) ![Dr. Raphael Nir](https://sbhsciences.com/hs-fs/hubfs/Imported%20sitepage%20images/Raphael_Nir.jpg?width=70&name=Raphael_Nir.jpg) ### Written By: Dr. Raphael Nir Dr. Raphael Nir has served as President and Chief Scientific Officer of SBH Sciences since co-founding the company in 1997. He started SBH Sciences to fulfill his dream of becoming a key player in the biotech arena, and to help companies develop drugs. Under his management, SBH Sciences has become a leading supplier of recombinant cytokines and a preferred discovery research service provider. ### Sign up for our newsletter! **Discovery Research Products & Services** (508) 650-6218 | [Inquiries@SBHsciences.com](mailto:Inquiries@SBHsciences.com) The World's Leading Provider of Cytokine Cell-Based Assays Recombinant Cytokines, Enzymes, Antibodies, Cell-Based Assay and Innovative Process Development Solutions **[COMPANY](https://sbhsciences.com/about-sbh)** [About Us](https://sbhsciences.com/about-sbh) [SBH Leadership](https://sbhsciences.com/sbh-leadership) [Company Presentation](https://sbhsciences.com/hubfs/43587181/SBH_Presentation.pdf) [Strategic Partnerships](https://sbhsciences.com/partnerships) [References and Publications](https://sbhsciences.com/references) [News and Blog](https://sbhsciences.com/sbh-blog-and-news) [Careers](https://sbhsciences.com/careers) [Request a Quote](https://sbhsciences.com/contact) [Contact Us](https://sbhsciences.com/contact) **[SERVICES](https://sbhsciences.com/services)** [Automated Western Blot](https://sbhsciences.com/platform-technologies#wb) [Bioanalytical](https://sbhsciences.com/bioanalytical-discovery-services) [Biomarker Testing](https://sbhsciences.com/biomarker-testing) [Cell-Based Assay](https://sbhsciences.com/cell-based-assay-services) [Cell Culture Services](https://sbhsciences.com/cell-culture-services) [Cell & Gene Therapy (CGT)](https://sbhsciences.com/cgt) [Small Molecule](https://sbhsciences.com/small-molecule) [Biosimilars](https://sbhsciences.com/biosimilars) [Antibody Development](https://sbhsciences.com/antibody-development-1) [ELISA Services](https://sbhsciences.com/elisa-assays) [Molecular Biology](https://sbhsciences.com/molecular-biology-services) [Organoids](https://sbhsciences.com/blog/organoids-mini-organs-grown-for-research-and-drug-development) [Exosomes](https://sbhsciences.com/exosome-services) [Protein Purification/Production](https://sbhsciences.com/protein-production-services) [FTE Scientist Model](https://sbhsciences.com/fte-model) [Consulting](https://sbhsciences.com/mentoring) **INDICATIONS** [Oncology](https://sbhsciences.com/oncology) [Kidney](https://sbhsciences.com/kidney) [Muscle](https://sbhsciences.com/muscle) [Metabolic](https://sbhsciences.com/metabolic) [Neurology](https://sbhsciences.com/neurology) **[PRODUCTS](https://sbhsciences.com/products)** [Request a Quote](https://sbhsciences.com/contact) [Cytokines](https://sbhsciences.com/cytokines) [Monoclonal Antibodies](https://sbhsciences.com/monoclonal-antibodies) [Glycobiology](https://sbhsciences.com/glycobiology) **[SBH Bioventure](https://sbhsciences.com/sbh-bioventure)** [**SBH Diagnostics**](https://sbhdiagnostics.com/#)   [![linkedin](https://sbhsciences.com/hs-fs/hubfs/Imported%20sitepage%20images/linkedin.gif?width=45&height=44&name=linkedin.gif)](https://www.linkedin.com/company/sbh-sciences)     ©2026 SBH Sciences. All rights reserved. [Terms of Use](https://sbhsciences.com/tou) | [Privacy Policy](https://sbhsciences.com/tou#pp) ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Dr. Raphael Nir", "url" : "https://sbhsciences.com/blog/author/dr-raphael-nir" }, "dateModified" : "2026-01-20T21:59:15.629Z", "datePublished" : "2025-03-25T18:30:38.000Z", "headline" : "High Throughput Screening: AlphaLISA (Part I)", "image" : [ "https://sbhsciences.com/hubfs/blog_sbh_author.jpg" ], "mainEntityOfPage" : { "@id" : "https://sbhsciences.com/blog/high-throughput-screening-alphalisa", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject", "url" : "https://sbhsciences.com/hubfs/Imported%20sitepage%20images/sbhlogo.png" }, "name" : "SBH Sciences" } } ``` ```json { "@context" : "https://schema.org", "@id" : "https://sbhsciences.com/blog/author/dr-raphael-nir#author", "@type" : "Person", "email" : "Inquiries@SBHsciences.com", "image" : { "@id" : "https://sbhsciences.com/blog/author/dr-raphael-nir#authoravatar", "@type" : "ImageObject", "url" : "https://43587181.fs1.hubspotusercontent-na1.net/hubfs/43587181/Imported%20sitepage%20images/Raphael_Nir.jpg" }, "name" : "Dr. Raphael Nir", "sameAs" : [ ] } ``` ```json { "@context" : "https://schema.org", "@id" : "https://sbhsciences.com/blog/high-throughput-screening-alphalisa#blogposting", "@type" : "BlogPosting", "author" : { "@id" : "https://sbhsciences.com/blog/author/dr-raphael-nir#author" }, "commentCount" : 0, "dateModified" : "2024-10-01T12:0000+0000", "datePublished" : "2025-03-25T18:3038+0000", "headline" : "High Throughput Screening: AlphaLISA (Part I)", "image" : "https://sbhsciences.com/blog/high-throughput-screening-alphalisa", "inLanguage" : "en", "keywords" : [ ], "mainEntityOfPage" : "https://sbhsciences.com/blog/high-throughput-screening-alphalisa", "name" : "High Throughput Screening: AlphaLISA (Part I)", "publisher" : { "@id" : "https://www.yourdomain.com#organization" }, "url" : "https://sbhsciences.com/blog/high-throughput-screening-alphalisa", "wordCount" : 589 } ```