Description
IL-23 is a proinflammatory heterodimeric protein composed of two subunits, a unique p19 subunit and a p40 subunit, which is shared with IL-12.IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ2. IL- 23 has been shown to enhance proliferation of memory T cells. It also stimulates the production of IFNgamma in NK cells, induces IL-17 production, and drives Th17 mediated responses. Recombinant IL-23 is a 53.5 kDa heterodimeric protein consisting of two subunits, p19 (170 amino acids) and p40 (306 amino acids).
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in 1x PBS pH 7.2-7.4 to a concentration of 0.1 mg/ml.
Storage: This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or at -20°C for future use.
| PROPERTIES | |
|---|---|
| Species | Human |
| Expression System |
High-5, Insect Cells |
| Molecular Weight | 53.5 kDa |
| Full Length | p19 subunit: 170 amino acids, p40 subunit: 406 amino acids |
| Purity |
≥95% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity |
Determined by the stimulation of IFN-gamma production by PBMC. The ED50 for this effect is < 10 -20 ng/ml, corresponding to a specific activity of >1 x 105 |
| Storage | -20°C |
