Description
Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+T cells and NK cells. Murine IL-12 also promotes the development of proinflammatory/TH1-like CD4+ cells and cytotoxic CD8+T cells. Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDA (p35) and 40 kDa (p40) subunits.
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration.
Storage: This solution can then be diluted into other aqueous buffers and stored at 4°C for 3 months or below -20°C for future use.
For research Purposes Only. Not for use in diagnostic or therapeutic procedures.
| PROPERTIES | |
|---|---|
| Species | Mouse |
| Expression System |
Chinese Hamster Ovary (CHO) Cells |
| Molecular Weight | 70 kDa |
| Full Length | p53 subunit: 391 amino acids |
| Purity |
≥95% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity | Determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-12. The expected ED50 for this effect is < 0.1 ng/ml, corresponding to a specific activity of >1 x107 units/mg |
| Storage | -20°C |