Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4R alpha is a 23.7 kDa protein containing 207 amino acid residues consisting of only the extracellular domain of the IL-4R alpha.
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml.
Storage: This solution can then be diluted into other aqueous buffers and stored at 4°C. for 1 week or -20°C. for future use.
For research Purposes Only. Not for use in diagnostic or therapeutic procedures.
| PROPERTIES | |
|---|---|
| Species | Human |
| Expression System |
Human Embryonic Stem Cells, HEK-293 |
| Molecular Weight | 23.7 kDa |
| Full Length | 207 amino acids |
| Purity |
≥97% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity | The ED50 as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is [5.0 ng/ml (in the presence of 0.5 ng/ml IL-4), corresponding to a specific activity of ] 2 x 10 exp5 units/mg |
| Storage | -20°C |