Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry
Product Specific Information
Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 50 ug/ml.
Storage: This solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2mg/ml albumin and stored at -80°C.
For research Purposes Only. Not for use in diagnostic or therapeutic procedures.
| PROPERTIES | |
|---|---|
| Species | Mouse |
| Expression System | High-5, Insect Cells |
| Molecular Weight | 28.9 kDa |
| Full Length | 227 amino acids |
| Purity |
≥98% by SDS-PAGE and HPLC Analyses |
| Form | Lyophilized |
| Endotoxin | <1 EU/µg |
| Activity | Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per ug of enzyme |
| Storage | -20°C |
