Description : |
Murine Hepatocyte Growth Factor is also known as Scatter Factor and Hepatopoeitin A. HGF is a potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. Recombinant Murine HGF is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (233 amino acids). |
Catalog #: |
003-mHGF |
Source : |
High-5 (BTI-Tn-5B1-4) Insect Cells |
Formulation : |
The sterile filtered solution was lyophilized from 0.1M L-Arganine HCl + 10 mM Tris, pH 8.0. |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted HGF should be stored in working aliquots at -200C. |
Purity : |
Greater than 98% by SDS-PAGE. Endotoxin level
is less than 0.1 ng per μg (1EU/μg).
|
Reconstitution : |
We recommend a quick spin followed by reconstitution in water to a concentration of 0.5 mg/ml. This solution can then be diluted into other buffers and stored at 40C for 1 week or stored at -200C for future use. |
Biological Activity : |
The ED50 was determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells was found to be in the range of 10.0-20.0 ng/ml. |
PubMed Link : |
HGF Hepatocyte Mouse |
Usage: |
For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures. |
Country of Origin : |
USA |
